About amphotericin-bit is a polyene antifungal antibiotic.indications for amphotericin-b1.oral, cutaneous, vaginal candidiasis 2.otomycosis 3.mucocutaneous candidiasis 4.fungal meningitis 5.kala azar 6.cryptococcosis 7.histoplasmosis 8.coccidioidomycosis 9.blastomycosis 10.paracoccidioidomycosis 11.aspergillosis 12.sporotrichosis 13.systemic mycoses mechanism of action of amphotericin-bamphotericin b has high affinity for ergosterol present in the fungal cell membrane and bind with it and they orients in such a way that micropore channel are formed and leads to efflux of ions, amino acid, sugar and other water soluble substances and results in cell disruption.