About ofloxacinsa second generation fluoroquinolone(quinolones), antibiotic.ssmechanism of action of ofloxacinsofloxacin is a bactericidal drug. it act by inhibiting the enzyme dna gyrase (topoisomerase 2) and topoisomerase 4.dna gyrase helps in the formation of a highly condensed three dimensional structure of the dna by its nicking and closing activity and also by introducing negative supercoil in to the dna double helix. ofloxacin inhibits dna gyrase which results in abnormal linkage between opened dna and gyrase and negative supercoiling is also impaired. this will inhibits transcription of dna in to rna and subsequent protein synthesis.sindications for ofloxacins1. bronchitis s2. pneumonia s3. urinary tract infection s4. bone and joint infections s5. intra-abdominal infections s6. gonorrhoea s7. ent infections s8. skin and soft tissue infections s9. typhoid fever s10. prostatis s11. otitis s12. gynaecological infections s13. conjunctivitis s14. keratitis s